Generator

Part:BBa_K1075051

Designed by: Max Schelski   Group: iGEM13_Bonn   (2013-10-12)


pLac-RBS34-mazF-TT - inducible Killswitch

This part consists of the Toxin mazF which is cappable of activating cell death. This Biobrick includes an inducible promoter (pLac) enabling us to IPTG inducible kill Bacteria. We tested this part with a weaker RBS (B0032) and a stronger RBS (B0034 this part). This part is cappable of inducing cell death in bacteria.

MazF is part of the biological toxin-antitoxin system mazEF. This system is derived from the bacterium Escherichia coli, where it serves as a stress inducable killswitch. mazF, a RNA-degrading enzyme, ist he toxin and is normally inhibited by the presence of mazE. Absence of mazE mazF leads to cell death.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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